Upright microscope

This configuration of the microscope has the objective above the sample rather than below. It is newer and less well tested than other versions of the microscope.

If you use this microscope for research please consider citing our paper in Optics Express.

For known bugs, building tips, and advice, please use our Forum. If you find any problems with the build, please let us know on GitLab or on the Forum.

Before you start building the microscope you will need to source all of the components listed in the bill of materials (HTML, CSV).

The assembly is broken up into several steps:

  1. Configure and test your printer
  2. Print the plastic parts
  3. Prepare the main body
  4. Prepare the separate z-actuator
  5. Prepare the upright z-actuator mount
  6. Prepare the microscope stand
  7. Assemble the actuators
  8. Assemble the basic optics module
  9. Assemble the illumination
  10. Mount the illumination and the microscope
  11. Mount the optics
  12. Assemble the motors
  13. Attach the sample clips
  14. Prepare the Raspberry Pi and Sangaboard
  15. Complete the wiring
  16. Assembly complete

A render of the completed upright microscope

There is also an interactive 3D view of the finished microscope.

These instructions assume you will build the upright microscope with a basic optics module. You can also use the high resolution optics module (with an RMS objective) on the upright microscope, or other options from the customisations page.

If using the high resolution optics module, a 400mm Raspberry Pi ribbon cable is preferable due to the additional height.


Next page