Assemble the high-resolution optics module

The imaging optics for this version of the microscope consist of an RMS objective, a tube lens and the Raspberry pi camera arranged as shown below (with the illumination optics)

Step 1: Visually inspect the optics module

Take the optics module and confirm that:

• It has been printed in black
• It is dust free (You can blow air through to clean it)
• The central shaft is not obstructed by strings of plastic

Step 2: Insert the tube lens

• Place the lens tool on a steady surface
• Place the 12.7 mm achromatic lens on the lens tool
• Check the lens has the more curved side facing down
• Take the optics module and place carefully over lens
• Push down hard to seat the lens in the optics module
• Visually inspect the positioning of the lens. It should be flat, not seated at an angle. If necessary, push against the lens tool to align properly

Step 3: Remove Pi Camera Lens

• Before touching the Pi Camera touch a metal earthed object. If you own one, consider wearing and anti-static strap.
• Take the Pi Camera out of the package. Make sure to hold it only by the sides of the board.
• Take the protective film off the lens.
• Take the Pi Camera lens tool and place it over the lens
• Slowly unscrew the lens (About 4 full turns of the tool)
• Carefully lift off the lens. We do not use the lens in this version of the microscope.

Step 4: Attach the Pi Camera

• Take the Pi Camera and place it on the back of the optics module
• Place the pi camera cover over the back of the Pi Camera.
• Use two No 2 6.5mm self tapping screws to secure the camera using a #1 pozidrive screwdriver
• Take care to not over torque the screws.

Step 5: Attach the objective

• Take your microscope objective and the partially assembled optics module
• Place objective on top of optics module
• Check that the objective is not tilted!
• Carefully and slowly screw the objective into the optics module, taking care to ensure it does not tilt.

Step 6: Attach the mounting screw

• Take an M3 nut and push it into the nut trap from the top
• Take an M3x10 cap head screws and screw it into the nut.
• Only screw it in a couple of turns. About 5 mm of thread should still be visible

Step 7: Connect ribbon cable

• Take the 200mm Pi Camera ribbon cable
• Pull the catch forward on the exposed Pi Camera connector
• Insert the ribbon cable with the contacts towards the board
• Close the catch on the connector

Step 8: Set the complete module aside

Set the complete optics module aside in a safe place.

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